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![可溶性白细胞介素2受体检测实验一夹心法ELISA]()
实验方法原理将抗Tac特异性单克隆抗体Ab1吸附于固相载体，识别细胞培养上清或血清等标本中Tac并与之结合。应用辣根过氧化物酶标记的识别另一种Tac表位的特异性单克隆抗体Ab2识别固定于固相载体的Tac并与之结合。通过酶催化底物显色反映待检...
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07082021实验步骤双抗体夹心ELISA法检测特异性抗体在有少量酶标特异抗体但无法获得纯化抗原时，用该检测方法筛选特异性抗体最为有效（图1.1.4)。另外，这种方法也可利用那些结合同一抗原的不同单克隆抗体来绘制抗原表位图。附加材料（其他材料见基本方案）包被抗体溶液（特异性针对待测种属免疫球蛋白的抗体，不结合抗原或标记抗体）碱性磷酸...
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15082021实验方法原理实验原理：以HCG作为抗原注入家兔体内，使其产生相应抗体，（抗HCG抗体）当这种抗体与抗原HCG相遇即可发生免疫反应，但此反应不能为肉眼所见采用化学方法将HCG交联在聚苯乙烯胶乳颗粒上制成HCG的胶乳抗原当此抗原与HCG抗体结合时就能够见到胶乳颗粒的凝集．试验时选用尿标本与抗血清充分混合，如果尿中含有大量H...268
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26122018st1:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable{mso-style-name:普通表格;mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-nos...167
